Real-time detection of airborne infection agents present in human breath and environmental airways, such as the human respiratory Coronavirus, is important for public health. For this, a model label-free immunosensor, based on multi-walled nanotubes (MWNT)-based screen-printed graphite electrodes (SPEs), was proposed and studied. For sensing applications, MWNTs have many advantages such as small size with larger surface area, excellent electron transfer promoting ability when used for antibody immobilization, with retention of its selectivity for potential immunosen-sors development. In order to verify the selectivity of the selected primary antibody (anti-CoV 229E antibody) and the effective immunocomplex formation (antigen-antibody interaction, an in-depth voltammetric characterization of MWNT-SPEs in-terface was carried out during the multistep fabrication of CoV immunosensor using [Fe(CN)6]3-/4- as an electroactive probe. After that, the analytical robustness of the performances of these immunosensing platforms was estimated and verified. Indeed, a nanomolar range detection limit (180 TCID50/mL)g/mL) with excellent reproduci-bility (RSD%=8%) was obtained.

Coronavirus Label-Free Immunosensor: Preliminary Results

Bellucci, S.;Betta, G.
Conceptualization
;
Ferrigno, L.
Methodology
;
Maffucci, A.
Methodology
;
Miele, G.
Methodology
2023-01-01

Abstract

Real-time detection of airborne infection agents present in human breath and environmental airways, such as the human respiratory Coronavirus, is important for public health. For this, a model label-free immunosensor, based on multi-walled nanotubes (MWNT)-based screen-printed graphite electrodes (SPEs), was proposed and studied. For sensing applications, MWNTs have many advantages such as small size with larger surface area, excellent electron transfer promoting ability when used for antibody immobilization, with retention of its selectivity for potential immunosen-sors development. In order to verify the selectivity of the selected primary antibody (anti-CoV 229E antibody) and the effective immunocomplex formation (antigen-antibody interaction, an in-depth voltammetric characterization of MWNT-SPEs in-terface was carried out during the multistep fabrication of CoV immunosensor using [Fe(CN)6]3-/4- as an electroactive probe. After that, the analytical robustness of the performances of these immunosensing platforms was estimated and verified. Indeed, a nanomolar range detection limit (180 TCID50/mL)g/mL) with excellent reproduci-bility (RSD%=8%) was obtained.
2023
978-3-031-25705-6
978-3-031-25706-3
File in questo prodotto:
File Dimensione Formato  
Proceeding AISEM RC LM ES SB 09-05-2022.pdf

non disponibili

Descrizione: post print
Tipologia: Documento in Post-print
Licenza: Non specificato
Dimensione 289.46 kB
Formato Adobe PDF
289.46 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11580/98087
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
social impact