It is well established that the nucleus is endowed with enzymes that are involved in lipid-dependent signal transduction pathways. Diacylglycerol (DAG) is a fundamental lipid second messenger that is produced in the nucleus. Previous reports have shown that the nucleus contains diacylglycerol kinases (DGKs), i.e., the enzymes that, by converting DAG into phosphatidic acid (PA), terminate DAG-dependent events. Here, we show, by immunofluorescence staining and confocal analysis, that DGK- localizes mainly to the nucleus of various cell lines, such as MDA-MB-453, MCF-7, PC12, and HeLa. Nuclear DGK- co-localizes with phosphatidylinositol 4,5-bisphosphate (PIP2) in domains that correspond to nuclear speckles, as revealed by the use of an antibody to the splicing factor SC-35, a well-established marker for these structures. The spatial distribution of nuclear DGK- was dynamic in that it was affected by inhibition of mRNA transcription with -amanitin. Immuno-electron microscopy analysis demonstrated that DGK-, PIP2, and phosphoinositide-specific phospholipase C1 (PLC1) associated with electron-dense particles within the nucleus that correspond to interchromatin granule clusters. Cell fractionation experiments performed in MDA-MB-453, HeLa, and PC12 cells showed a preferential association of DGK- with the nucleus. Western blots demonstrated that DGK- was enriched in the nuclear matrix fraction prepared from MDA-MB-453 cells. Immunoprecipitation experiments with an antibody to PLC1 revealed in MDA-MB-453 cells an association between this enzyme and both DGK- and phosphatidylinositol phosphate kinase I (PIPKI). Our findings strengthen the contention that speckles represent a crucial site for the nuclear-based inositol lipid cycle. We may speculate that nuclear speckle-located DGK-, on cell stimulation with an agonist, converts to PA the DAG derived from PLC1-dependent PIP2 hydrolysis.
Diacylglycerol kinase-theta is localized in the speckle domains of the nucleus.
CAPPELLINI, Alessandra;
2003-01-01
Abstract
It is well established that the nucleus is endowed with enzymes that are involved in lipid-dependent signal transduction pathways. Diacylglycerol (DAG) is a fundamental lipid second messenger that is produced in the nucleus. Previous reports have shown that the nucleus contains diacylglycerol kinases (DGKs), i.e., the enzymes that, by converting DAG into phosphatidic acid (PA), terminate DAG-dependent events. Here, we show, by immunofluorescence staining and confocal analysis, that DGK- localizes mainly to the nucleus of various cell lines, such as MDA-MB-453, MCF-7, PC12, and HeLa. Nuclear DGK- co-localizes with phosphatidylinositol 4,5-bisphosphate (PIP2) in domains that correspond to nuclear speckles, as revealed by the use of an antibody to the splicing factor SC-35, a well-established marker for these structures. The spatial distribution of nuclear DGK- was dynamic in that it was affected by inhibition of mRNA transcription with -amanitin. Immuno-electron microscopy analysis demonstrated that DGK-, PIP2, and phosphoinositide-specific phospholipase C1 (PLC1) associated with electron-dense particles within the nucleus that correspond to interchromatin granule clusters. Cell fractionation experiments performed in MDA-MB-453, HeLa, and PC12 cells showed a preferential association of DGK- with the nucleus. Western blots demonstrated that DGK- was enriched in the nuclear matrix fraction prepared from MDA-MB-453 cells. Immunoprecipitation experiments with an antibody to PLC1 revealed in MDA-MB-453 cells an association between this enzyme and both DGK- and phosphatidylinositol phosphate kinase I (PIPKI). Our findings strengthen the contention that speckles represent a crucial site for the nuclear-based inositol lipid cycle. We may speculate that nuclear speckle-located DGK-, on cell stimulation with an agonist, converts to PA the DAG derived from PLC1-dependent PIP2 hydrolysis.File | Dimensione | Formato | |
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