Enhanced lignocellulosic component removal and biomethane potential from chestnut shell by a combined hydrothermal-alkaline pretreatment

: This study proposes new perspectives for the management and biorefinery of wastes deriving from the agri-food sector such as chestnut shell (CS), which was here used as an organic feedstock for biomethane production through anaerobic digestion (AD). 1-5% alkaline (i.e. NaOH and KOH), hydrothermal (i.e. at 100 °C) and combined hydrothermal-alkaline pretreatments were employed to enhance the CS biodegradability prior to biochemical methane potential (BMP) tests conducted under mesophilic conditions. The hydrothermally-pretreated CS with 3% NaOH achieved the highest biomethane yield of 253 (±9) mL CH4·g VS-1 coupled to a volatile solid reduction of 48%. The hydrothermal-alkaline pretreatment positively affected both delignification and hemicellulose polymerization, promoting an approximately 2.4-fold higher substrate biodegradability compared to the untreated CS, which only reached a CH4 production of 104 (±5) mL CH4·g VS-1. AD proceeded via volatile fatty acid accumulation, subsequently followed by methane production that was effectively simulated via the modified Gompertz kinetic having a R2 of 0.974-0.999. Among the physical-chemical parameters characterizing the CS, the soluble chemical oxygen demand (sCOD) was highly correlated with the BMP showing a Pearson coefficient of 0.952. The cumulative biomethane yield, the sCOD and the cellulose, hemicellulose and lignin amount of CS were also processed through the least square method, obtaining a useful regression equation to predict the BMP. The economic assessment indicated that the hydrothermal-alkaline pretreatment is a cost-effective method to improve the BMP of CS, also for future full-scale applications.